Contact an Account ManagerDetermination of plasma norepinephrine and epinephrine by HPLC using a two-column system and an electrochemical detector
Summary
Type: HPLC
Application #: 101830
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: electrochemical, glassy carbon electrode at 0.75V versus Ag/AgCl
Conditions: eluent: 15g/l phosphate buffer (NaH2PO4·2 H2O), 300mg/l heptanesulphonate and 200mg/l EDTA, adjusted to pH 4.2 with 3M phosphoric acid,
flow rate 1ml/min identical for both pumps
Authors: Lachâtre, G. et al.
Source: J. Liquid Chromatogr. 5 (1982) 1947–1965
Details
Legend: Injection of 1ng of each catecholamine and 0.8ng of internal standard, in 200µl
0.1M HClO4. 1B. Chromatogram of catecholamines obtained from 4ml of human
plasma to which 200µl of a 8ng/ml DHBA solut